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Monthly Archives: February 2016

802, 2016

E-Juice/E-Liquid Product Testing Analysis & Safety Information

February 8th, 2016|Blog|

eliquid and ejuice testing

Over the course of the past 2 years consumer demand for e-liquids and e-cigarettes has been on the rise. During this time period there has been many concerns from the general public about how safe these e-liquids are and what ingredients they are comprised of.

Common ingredients in e-cigarettes and e-liquids include nicotine, vegetable glycerin, propylene glycol, water, natural and artificial flavoring. Flavoring either natural or artificial is commonly used in e-liquids to represent a food or specific flavor such as traditional tobacco, honey, cereal, mint, and more.

Nicotine-containing liquid restrictions:
• EC must not contain more than 20 mg/ml of nicotine
• nicotine-containing liquid must be in dedicated refill containers not exceeding 10ml volume, and cartridges or tanks do not exceed a volume of 2ml
• additives are not prohibited but the nicotine-containing liquids cannot contain additives that are otherwise prohibited by the other Articles in the TPD
• high purity ingredients must be used and substances other than those declared should only be present in trace quantities which are unavoidable during manufacture
• ingredients must not pose a risk to health either when heated or not heated
• nicotine doses must be delivered at consistent levels under normal conditions of use
• products are required to be child and tamper proof, protected against breakage and leakage and have a mechanism that ensures refilling without leakage

Products must include a leaflet with information on:
• instructions for use and storage of the product, including a reference that the product is not recommended for use by young people and non-smokers
• contra-indications
• warnings for specific groups
• possible adverse effects
• addictiveness and toxicity
• possible allergy information

Current E-Liquid Safety Concerns:
The primary purpose of our analysis on e-liquid is for safety concerns and public health. Manufacturers and distributors of e-liquids (or e-juice) should be aware of what is in their product. Many safety concerns about contents of e-liquids have been constructed (and some fabricated). You should know what you’re selling to your customers.

Indoor Air Quality
Many studies have been performed for indoor air quality of e-cigarette vapor. A main question and voiced concern for the public is how second-hand vapor affects us. Please see the information below:

Comparison of the Effects of E-cigarette Vapor and Cigarette Smoke on Indoor Air Quality

Levels of selected carcinogens and toxicants in vapor from electronic cigarettes

Does e-cigarette consumption cause passive vaping?

Electronic cigarettes: an evaluation of exposure to chemicals and fine particulate matter (PM)

Secondhand Exposure to Vapors From Electronic Cigarettes

What Am I Inhaling?
As mentioned above, there are common ingredients for most e-liquids. What adverse effects can be found from these substances?

Propylene Glycol:
Propylene Glycol or (PG) is one of the primary ingredients in e-cigarettes. According to research studies dating back to the 1940’s, this ingredient has been used in inhalants for quite some time in devices such as asthma inhalers, air disinfectants in hospitals and restaurants, and entertainment centers as well such as theaters, bars, and restaurants. This substance is also a main ingredient of fog machines.

Is PG Safe? Should I be concerned?
“Propylene glycol is used in air sanitization and hard surface disinfection and dipropylene glycol is used in air sanitization.”
“Propylene glycol and dipropylene glycol were first registered in 1950 and 1959, respectively, by the FDA for use in hospitals as air disinfectants.”
“Indoor Non-Food: Propylene glycol is used on the following use sites: air treatment (eating establishments, hospital, commercial, institutional, household, bathroom, transportation facilities); medical premises and equipment, commercial, institutional and industrial premises and equipment; laundry equipment; hard non-porous surface treatments (bathroom facilities); automobiles; air conditioning filters; pet treatment, including cats, dogs, and caged birds; environmental inanimate hard surfaces; garbage containers/storage.”
“Target Pests: Odor-causing bacteria, Fleas, Mites, Red lice, Animal pathogenic bacteria (G- and G+ vegetative), Shigella bacteria, Pasteurella bacteria, Listeria bacteria, Herpes Simplex I and II, Animal viruses, Influenza Virus A2, Aspergillus Niger Fungus, Mold/Mildew, Pseudomonas SPP., Shigella Flexneri, Shigella Sonnei.”
General Toxicity Observations
“Upon reviewing the available toxicity information, the Agency has concluded that there are no endpoints of concern for oral, dermal, or inhalation exposure to propylene glycol and dipropylene glycol. This conclusion is based on the results of toxicity testing of propylene glycol and dipropylene glycol in which dose levels near or above testing limits (as established in the OPPTS 870 series harmonized test guidelines) were employed in experimental animal studies and no significant toxicity observed.”

Carcinogenicity Classification
“A review of the available data has shown propylene glycol and dipropylene glycol to be negative for carcinogenicity in studies conducted up to the testing limit doses established by the Agency; therefore, no further carcinogenic analysis is required.”

And there is much more information in the full document linked below:

Vegetable Glycerin:
Vegetable Glycerin or (VG) is also a main component used in e-liquids. VG is the substance in an e-liquid that makes the liquid thicker. It is a carbohydrate that is usually derived from plant oils. This substance is used as a sweetener in many foods as well. Most e-liquid providers now use USP Grade VG rather than Food Grade.

VG is also used in place of alcohol for many extracts. See:
Products with Vegetable Glycerin can be found in various common items around your house. A few examples include:
• Sugar substitute
• In Beauty products including makeup, mousse, shampoo, bubble bath, after shave, and deodorant
• Pet food
• Soap
• Skin and hand cream
• Baked goods – increase moisture
• As a thick gel for creams, gel capsule pills, rubs and jellies
• Eye & ear drops, toothpastes, pastes, and many dental care products

Glycerine is classified by the U.S. Food and Drug Administration (FDA) as “generally recognized as safe” (GRAS) and complies with specifications for the Food Chemicals Codex (FCC), United States Pharmacopeia (USP), and European Pharmacopoeia (Ph. Eur. or EP) E244. It is manufactured according to current Good Manufacturing Practices (cGMP) and is shipped according to applicable Good Trade and Distribution Practices (GTDP). See:

For more information on scientific and medical information on e-liquid and electronic cigarettes, please visit this webpage:

What is the difference between USP Grade and Pharmaceutical Grade?
USP Grade materials allows for fillers and binders added during the manufacturing process, and the additives don’t have to be on the label per the FDA. Common fillers are things like corn sugars, and propleyene Glycol.

Pharmaceutical grade means the product was made using GMP, or Good Manufacturing Practices, and has to be 98-99.7% pure. For more information, please see:

How Can Avomeen Help?
Avomeen’s laboratory performs a verity of e-liquid testing and development. Our chemical analysis can help you determine if your product is safe for consumption. Our labs include:
• Determine e-liquid recipes through chemical reverse engineering (if you wish to have a competitive edge on other distributors)
• Deformulation of e-liquid flavor profiles & identification of flavoring agents or additives used within a product’s formulation. We can help achieve the proper flavor with less additives.
• Carcinogenic analysis including toxic ingredient analysis for Diacetyl, Propinyl Acetyl, and Acetoin
• Analyze e-liquids for unknown ingredients and determine the purity and grade of its raw ingredients
• Test your e-liquid for nicotine levels to formulate accuracy (USP Assay)
• Metals VOC Analysis
• VG/PG ratio
• Safety Data Sheet
• Toxicology Testing – Verify the safety of your product
• Determine products shelf life under various storage conditions and give recommendations for storage and expiration for your customers
• Develop new custom formulation of e-liquid recipes

These services are primarily utilized by and conducted for manufacturers and distributors of e-liquid products. If you manufacture or distribute e-liquid products and you would like analysis done for safety, legal, buy confidence or any other purposes, give us a call for a free consolation with one of our PhD chemists. We have a full service chemical testing laboratory and can provide the e-liquid testing you desire!

202, 2016

Ink Manufacturing & Contaminant Risk

February 2nd, 2016|Blog|

Ink Manufacturing & Testing
Paint and ink manufacturing has been through dramatic changes due to a call for environmental and consumer protection awareness. Research surrounding harmful contaminants and their use has influenced government regulation, embodied by the Food & Drug Administration (FDA) and the Centers for Disease Control (CDC) in the United States, in most industries with the purpose of creating an informed public. The most common example in the paint and ink industry would be the ban of lead pigment-based paints in 1978. A toxic heavy metal, lead was found to negatively affect most systems in the body, especially the nervous system, over prolonged exposure causing cancer and other complications. The further discovery of materials that can contaminate or alter consumer paints and inks has led to the need for quality control as well as research and development devoted to more conscious products.

The possible contaminants found in the process of ink production is made up of ingredients, byproducts, and other materials the final product may come in contact with during manufacturing:
• Metals, such as chromium, cadmium, lead, and zinc
• VOCs (Volatile Organic Compounds)
• Chloroform
• Ethyl Benzene
• Various Solvents
• Residual Paint or Ink
• Bacteria, etc.

Most are not easily detectable due to their chemical properties, and require specialized processes to determine presence and levels. Bacteria were the subject of recent discussion surrounding tattoo ink in 2014 when tattoo recipients noticed symptoms such as fevers, rashes, and excessive pain. A California company was forced to recall when tests found evidence of bacteria in unopened bottles of ink. The FDA further warned that this had not been, and would not be, the only case of contaminated ink affecting consumers, and manufacturers.

Contaminated paints and inks present both potential health concerns and business-related obstacles. The list above contains multiple carcinogens and allergens harmful to the human body and the immediate environment. Each can be assumed to have properties that affect normal bodily processes in humans, at the very least causing headaches and skin irritation. Also, the potential for negative environmental consequences, especially in the form of waste, is noteworthy as it still directly affects the general population.

There are risks for manufacture companies in the industry as well. Clean up costs after the fact, including public relations, are the first to come to mind. But it is the quality of the product that is undermined by potential impurities. In an industry that already worries about the effect of the product on the consumer, maintaining a consistent product is important to longevity, both in profit and usefulness of a product. Consequently, taking the time to find impurities in a paint or ink product before market is an essential step in preventing recalls or other similar outcomes.
Contamination testing provides the answer in identifying a contaminant, if any, and in helping to find possible solutions for future batches. By adding a step for quality control testing into the overall production process, likely useless batches can be reversed more quickly allowing a manufacturer to minimize potentially harmful or low quality products to enter the market. At Avomeen our staff is skilled in various research methods, such as chemical testing, that help locate impurities to their smallest presence. Our results will give you the opportunity to make informed decisions in the future of your paint and ink production.